| Date | Home | Away | Result |
|---|---|---|---|
| 31/10/25 19:30 | Luton Town | Forest Green Rovers | --- |
| 01/11/25 12:00 | Chelmsford City | Braintree Town | --- |
| 01/11/25 15:00 | Wycombe Wanderers | Plymouth Argyle | --- |
| 01/11/25 15:00 | Halifax Town | Exeter City | --- |
| 01/11/25 15:00 | Boreham Wood | Crawley Town | --- |
| 01/11/25 15:00 | Bolton Wanderers | Huddersfield Town | --- |
| 01/11/25 15:00 | Oldham Athletic | Northampton Town | --- |
| 01/11/25 15:00 | Crewe Alexandra | Doncaster Rovers | --- |
| 01/11/25 15:00 | Stevenage FC | FC Chesterfield | --- |
| 01/11/25 15:00 | Barnsley FC | York City | --- |
| 01/11/25 15:00 | Reading FC | Carlisle United | --- |
| 01/11/25 15:00 | Bromley FC | Bristol Rovers | --- |
| 01/11/25 15:00 | Peterborough United | Cardiff City | --- |
| 01/11/25 15:00 | AFC Wimbledon | FC Gateshead | --- |
| 01/11/25 15:00 | Mansfield Town | Harrogate Town FC | --- |
| 01/11/25 15:00 | Fleetwood Town | FC Barnet | --- |
| 01/11/25 15:00 | FC Blackpool | Scunthorpe United | --- |
| 01/11/25 15:00 | Wealdstone FC | Southend United | --- |
| 01/11/25 15:00 | Rotherham United | Swindon Town | --- |
| 01/11/25 15:00 | Grimsby Town | Ebbsfleet United | --- |
| 01/11/25 15:00 | Tranmere Rovers | Stockport County | --- |
| 01/11/25 15:00 | Wigan Athletic | Hemel Hempstead Town | --- |
| 01/11/25 15:00 | Newport County | FC Gillingham | --- |
| 01/11/25 15:00 | Cheltenham Town | Bradford City | --- |
| 01/11/25 15:00 | Salford City | Lincoln City | --- |
| 01/11/25 15:00 | Colchester United | Milton Keynes Dons FC | --- |
| 01/11/25 15:00 | Weston-Super-Mare | Aldershot Town | --- |
| 01/11/25 15:00 | Spennymoor Town | Barrow FC | --- |
| 01/11/25 15:00 | Slough Town FC | Altrincham FC | --- |
| 01/11/25 15:00 | Sutton United | Telford United | --- |
| 01/11/25 15:00 | Burton Albion | St Albans City | --- |
| 01/11/25 15:00 | Cambridge United | Chester FC | --- |
| 01/11/25 15:00 | Buxton FC | Chatham Town | --- |
| 01/11/25 15:00 | Macclesfield FC | AFC Totton | --- |
| 01/11/25 17:30 | Brackley Town | Notts County | --- |
| 02/11/25 12:00 | South Shields FC | Shrewsbury Town | --- |
| 02/11/25 14:15 | Eastleigh FC | FC Walsall | --- |
| 02/11/25 15:00 | FC Port Vale | Maldon and Tiptree FC | --- |
| 02/11/25 17:15 | Gainsborough Trinity | Accrington Stanley | --- |
| 03/11/25 19:30 | FC Tamworth | Leyton Orient | --- |