| Date | Home | Away | Result |
|---|---|---|---|
| 05/12/25 19:30 | Salford City | Leyton Orient | 4-0 |
| 06/12/25 15:00 | Exeter City | Wycombe Wanderers | 4-0 |
| 06/12/25 15:00 | Cheltenham Town | Buxton FC | 6-2 |
| 06/12/25 15:00 | Accrington Stanley | Mansfield Town | 3-5a.PSO |
| 06/12/25 15:00 | Stockport County | Cambridge United | 4-5a.PSO |
| 06/12/25 15:00 | Milton Keynes Dons FC | Oldham Athletic | 3-1 |
| 06/12/25 15:00 | Wigan Athletic | Barrow FC | 6-5a.PSO |
| 06/12/25 15:00 | Fleetwood Town | Luton Town | 6-4a.PSO |
| 06/12/25 15:00 | Swindon Town | Bolton Wanderers | 4-0 |
| 06/12/25 15:00 | Chelmsford City | Weston-Super-Mare | 0-2 |
| 06/12/25 15:00 | Peterborough United | Barnsley FC | 0-1 |
| 06/12/25 15:00 | Grimsby Town | Wealdstone FC | 4-0 |
| 06/12/25 15:00 | FC Port Vale | Bristol Rovers | 1-0 |
| 06/12/25 17:15 | Sutton United | Shrewsbury Town | 1-2a.e.t |
| 06/12/25 19:30 | FC Chesterfield | Doncaster Rovers | 1-2 |
| 07/12/25 12:30 | Slough Town FC | Macclesfield FC | 1-3a.e.t |
| 07/12/25 14:30 | Boreham Wood | Newport County | 3-0 |
| 07/12/25 15:30 | FC Gateshead | FC Walsall | 0-2 |
| 07/12/25 17:30 | FC Blackpool | Carlisle United | 4-1 |
| 08/12/25 19:30 | Brackley Town | Burton Albion | 1-3 |